DR Mersimine KOUAMO

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Background

Msc in Biochemistry University of Yaoundé I, Cameroon on “Evaluation of antioxidant and anti-inflammatory activities of polysaccharide fractions of Khaya grandifoliola C.D.C stem bark.”

Member of COACH Cameroon

Member of biostrctural Africa working group

– Reviewer for research journals

Asian Journal of Research in Zoology

Asian Journal of Advances in Agricultural Research

Asian Journal of Agricultural Extension, Economics & Sociology

International Journal of Environment and Climate Change

Neglected Tropical Diseases

Pesticide Biochemistry and Physiology

Asian Journal of Research in Nursing and Health

Asian Journal of Medicine and Health

Asian Journal of Advances in Research

Asian Journal of Research in Biochemistry

Open journal of tropical medicine

Research:

Areas of interest:
Functional validation of mosquito’s insecticide resistance/candidate gene Entomology/ Molecular biology/ Medical Entomology.
Research – an overview of your research areas(s)

Entomology: Collection of mosquitoes on the field, morphological and molecular Identification, Mapping, drosophila and mosquito rearing in the insectary.

molecular biology technology: As PCR, ELISA, DNA and RNA extraction, molecular cloning…

Functional genomic technics: RNA interference, transgenic expression of candidate gene in insecticide resistance, gene expression, gene editing, proteomic.

Gene expression: expression profile, ARN sequencing and pooseq.

Detect resistant marker: allelic variation, genotyping.

Bioinformatics analysis : use of motors search (Agora, Oare, Pudmed et Hinari) use of some molecular analysis software such as Molegro, Pymol and modeler for structural characterization of protein; vector base, fly base NCBI, DNASP, CLC sequence vieuwer, Biodit, Mega script, vasastat, Primers 3, SPSS, FlowJo (to analyses flow cytometry result).

Characterisation of drosophila melanogaster D. erecta in Cameroon.

Other Relevent Expertise, PROFESSIONAL MEMBERSHIPS

– Membership

Member of Pan-African Mosquitoes Association (PAMCA)

Member of women in Malaria (WIM)

Member of Malaria Vector Control Working

Member of Cameroon society of des Biosciences

Member of African Education Initiative (NEF)

PhD students
Current students bench supervisor at CRID under Prof Charles Wondji supervision

DJOKO Carlos

TCHATCHOUA Nelly

NGANANG Vanessa

Tepa Arnaud

TEKOH Theofelix

BAKAM Larissa

NDIFO Blanche

NGO Amy
Publications

Kouamo M.F.M.; Ibrahim S.S.; Hearn J.; Riveron J.M.; Kusimo M.; Tchouakui M.; Ebai T.; Tchapga W.; Wondji M.J.; Irving H.; Boudjeko T.; Boyom F.F.; Wondji C.S.. Genome-Wide Transcriptional Analysis and Functional Validation Linked a Cluster of Epsilon Glutathione S-Transferases with Insecticide Resistance in the Major Malaria Vector Anopheles funestus across Africa. Genes 2021, 12, 561.

Ibrahim S. S., Kouamo, M. F., Muhammad A., Irving H., Riveron J. M., Tchouakui M., & Wondji C. S. (2024). Functional Validation of Endogenous Redox Partner Cytochrome P450 Reductase Reveals the Key P450s CYP6P9a/-b as Broad Substrate Metabolizers Conferring Cross-Resistance to Different Insecticide Classes in Anopheles funestus. International Journal of Molecular Sciences, 25(15), 8092.

Djoko Tagne, C. S., Kouamo, M. F., Tchouakui, M., Muhammad, A., Mugenzi, L. J., Tatchou-Nebangwa, N. M., … & Wondji, C. S. (2024). A single mutation G454A in P450 CYP9K1 drives pyrethroid resistance in the major malaria vector Anopheles funestus reducing bed net efficacy. bioRxiv, 2024-04.

Menze B.D.; Kouamo, M.F.; Wondji, M.J.; Tchapga, W.; Tchoupo, M.; Kusimo, M.O.; Mouhamadou, C.S.; Riveron, J.M.; Wondji C.S. An Experimental Hut Evaluation of PBO-Based and Pyrethroid-Only Nets against the Malaria Vector Anopheles funestus Reveals a Loss of Bed Nets Efficacy Associated with GSTe2 Metabolic Resistance. Genes 2020, 11, 143.

KENGNE-OUAFO, Jonas A., KOUAMO Mersimine, MUHAMMAD Abdullahi, et al. A single E205D allele of a key P450 CYP6P3 is driving metabolic pyrethroid resistance in the major African malaria vector Anopheles gambiae. bioRxiv, 2024, p. 2024.02. 18.580859.

Tchouakui M., Ibrahim S. S., Mangoua M. K., Thiomela R. F., Assatse T., Ngongang-Yipmo S. L., … & Wondji C. S. (2024). Substrate promiscuity of key resistance P450s confers clothianidin resistance while increasing chlorfenapyr potency in malaria vectors. Cell reports, 43(8).

Tatchou-Nebangwa N. M., Mugenzi L. M., Muhammad A., Nebangwa D. N., Kouamo M. F., Tagne C. S. D., … & Wondji, C. S. (2024). Two highly selected mutations in the tandemly duplicated CYP6P4a and CYP6P4b genes drive pyrethroid resistance in Anopheles funestus in West Africa. BMC biology, 22, 286.

Mugenzi L. M., A. Tekoh T., S. Ibrahim S., Muhammad A., Kouamo M., Wondji M. J., … & Wondji, C. S. (2023). The duplicated P450s CYP6P9a/b drive carbamates and pyrethroids cross-resistance in the major African malaria vector Anopheles funestus. PLoS Genetics, 19(3), e1010678.

SIMO, Marguerite Kamdem, SIWE, Gael Tchokomeni, TABOULA KAYO, Maurice, M, Mangoua Mersimine et al. Anti-inflammatory, Antinociceptive, and Toxicological Properties of Uvaria comperei Stem Crude Extract and Fractions. BioMed Research International, 2023, vol. 2023.